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Here, we apply MISC to the study of a very important public health problem: bacterial antibiotic resistance. Specifically, we have obtained time-resolved crystallographic data on the binding and cleavage of the third-generation antibiotic ceftriaxone (CEF) in microcrystals of the enzyme β-lactamase from Mycobacterium tuberculosis (BlaC). Previous studies introduced mutations into BlaC by exchanging catalytically important residues to slow down (or stop) the reaction to the extent that the binding of numerous antibiotics to BlaC could be studied [39]. In our experiments, however, carried out at the Linac Coherent Light Source (LCLS), microcrystals of unmodified BlaC are mixed with CEF on the fly, and the cleavage and thereby inactivation of the antibiotics by the wild-type β-lactamase is followed at runtime. BlaC is a broad-spectrum β-lactamase which confers resistance to all classes of β-lactam antibiotics in tuberculosis [19, 40]. BlaC chemistry has rendered the frontline arsenal of antibacterial agents ineffective against this deadly disease, creating a global public health crisis.
The structures and diffraction data of BlaC, unmixed and mixed with ceftriaxone at 0 s, 30 ms, 100 ms, 500 ms, and 2 s for shard and needle crystal forms are deposited to the Protein Data Bank (PDB) with the following access codes: Shards 6B5X (unmixed), 6B5Y (30 ms), 6B68 (100 ms), 6B69 (500 ms), 6B6A (2 s); needles 6B6B (unmixed), 6B6C (30 ms), 6B6D (100 ms), 6B6E (500 ms), 6B6F (2 s). None of the authors declare competing interests. Clones and other material such as computer code are available on request from the corresponding author.
Understanding the different concrete and cement types ensures you'll reach for the right product for every project. Whether you're repairing a cracked sidewalk, extending an outdoor patio or simply setting new fence posts, read and follow the directions on your product so you'll know how to mix it and how long to let it dry and cure. Looking for a tool, material or other item Use our The Home Depot Mobile App to search by voice or image.
Preblended Cement Base CoatStandard (SU-05)PRODUCT DATA SHEETTECHNICAL DOWNLOADSPROJECT PROFILESFIND A DEALERCONTACT REPRESENTATIVEEXCELLENT WORKABILITY. SUPERIOR PRODUCTION.Product FeaturesComputer batched for consistency and performance unmatched by traditional field mixed stucco.Custom blended sand gradation that increases the wall flexural strength while reducing cracking and crazing.Excellent workability an finishing properties for the applicator over a traditional field-mixed sourceReduced shrinkage and increases long term durability and bond strength of the applicationSPEC MIX Fiber Base Coat (FBC) is a dry, preblended cement based stucco engineered with proprietary admixtures and fibers that are computer batched for consistency and performance unmatchedby traditional field mixed stucco. SPEC MIX FBC has a custom blended sand gradation that increases the wall flexural strength while reducing cracking and crazing. The custom sand gradation also provides excellent workability and finishing properties for the applicator over a traditional field-mixed source.
Generally, pavement overlays are used to restore surface course (both HMA and PCC) characteristics (such as smoothness, friction and aesthetics) or add structural support to an existing pavement. However, even a structural overlay needs to be placed on a structurally sound base. If an existing pavement is cracked or provides inadequate structural support these defects will often reflect through even the best-constructed overlay and cause premature pavement failure in the form of cracks and deformations. To maximize an overlays useful life, failed sections of the existing pavements should be patched or replaced and existing pavement cracks should be filled.
Existing pavement crack repair methods depend upon the type and severity of cracks. Badly cracked pavement sections, especially those with pattern cracking (e.g., fatigue cracking) or severe slab cracks, must be patched or replaced because these distresses are often symptoms of more extensive pavement or subgrade structural failure (TRB, 2000[1]). Existing cracks other than those symptomatic of structural failure should be cleaned out (blown out with pressurized air and/or swept) and filled with a crack-sealing material when the cracks are clean and dry (TRB, 2000[1]). Cracks less than about 10 mm (0.375 inches) in width may be too narrow for crack-sealing material to enter. These narrow cracks can be widened with a mechanical router before sealing. If the existing pavement has an excessive amount of fine cracks but is still structurally adequate, it may be more economical to apply a general bituminous surface treatment (BST) or slurry seal instead of filling each individual crack.
Placing a HMA overlay on a jointed or cracked PCC pavement involves some special considerations in addition to the usual repair and leveling. Jointed plain concrete pavement (JPCP) is placed in discrete slabs and both JPCP and continuously reinforced concrete pavement (CRCP) tend to crack into discrete sections.These slabs/sections tend to move as individual units. Although HMA overlays can accommodate small differential subgrade movement without cracking, the large differential movement at slab and crack interfaces is great enough to crack a HMA overlay (called reflection cracking). There are several techniques to prevent (or at least delay the onset of) reflection cracking:
Given the expense of these techniques, some agencies just choose to live with joint reflection cracking rather than prevent it. This is especially true on low volume, low speed roads where ride smoothness and structural integrity may not be given the high priority they are on high volume, high speed roads like interstates.
Mixing your concrete with reinforcing fibers and using reinforcing rods laid down on a 12-in. x 16-in. grid pattern greatly reduces the chance of crack formation. Also, two weeks after pouring, use a masonry saw to make cuts one-third of the way through the thickness of your concrete slab. Make these cuts in a 10-ft. x 10-ft. grid pattern. Any slight cracks that may form will follow the saw cuts and be hidden by them.
Too few DIYers know about concrete reinforcing fibers. These thin, short strands of plastic add a lot of strength and crack resistance to any kind of concrete project. Add a pint of fibers to each mixing drum load of concrete and mix as usual. The fibers spread out within the mix and help bind the hardened concrete together. They make a big difference.
Cool Whip cookies use a box cake mix as well, but Cool Whip whipped topping replaces the oil, resulting in a softer cookie with a different texture. Cool Whip cookie dough is sticky, so the cookie dough balls are rolled in powdered sugar giving them a cracked crinkle cookie appearance when baked.
Continue to Content{\"@context\":\"http:\\/\\/schema.org\",\"@type\":\"Recipe\",\"name\":\"Lemon Cool Whip Cookies\",\"author\":{\"@type\":\"Person\",\"name\":\"TidyMom\"},\"datePublished\":\"2019-02-22\",\"recipeYield\":24,\"description\":\"Enjoy the burst of lemon flavor from these light, fluffy soft baked Lemon Whippersnaps.\",\"image\":[\"https:\\/\\/tidymom.net\\/blog\\/wp-content\\/uploads\\/2021\\/03\\/lemon-cool-whip-cookies-pic.jpg\"],\"recipeCategory\":\"Cookies\",\"recipeCuisine\":\"American\",\"prepTime\":\"PT15M\",\"cookTime\":\"PT12M\",\"performTime\":\"PT12M\",\"totalTime\":\"PT27M\",\"recipeIngredient\":[\"1 box Lemon* Cake Mix (preferably with pudding in the mix)\",\"1 (8 ounce) container Cool Whip, completely thawed\",\"1 large egg, beaten\",\"1\\/2 cup powdered sugar (for rolling)\"],\"recipeInstructions\":[{\"@type\":\"HowToStep\",\"text\":\"Preheat oven to 350\\u00b0 F and line a\\u00a0half sheet pan\\u00a0or cookie sheet with parchment paper or a silicone baking sheet.\",\"position\":1,\"name\":\"Preheat oven to 350\\u00b0 F and line a\\u00a0half...\",\"url\":\"https:\\/\\/tidymom.net\\/lemon-cool-whip-cookies\\/#mv_create_171_1\"},{\"@type\":\"HowToStep\",\"text\":\"In a medium bowl combine dry cake mix, and beaten egg. Mixture will be crumbly. Fold in thawed Cool Whip until well combined. Dough will be thick and sticky.\",\"position\":2,\"name\":\"In a medium bowl combine dry cake mix,...\",\"url\":\"https:\\/\\/tidymom.net\\/lemon-cool-whip-cookies\\/#mv_create_171_2\"},{\"@type\":\"HowToStep\",\"text\":\"Using a\\u00a0small cookie scoop\\u00a0(about 1.5 tablespoon scoop) drop the dough balls into a bowl of powdered sugar, and roll to coat. Place the dough balls onto the prepared cookie sheet about two inches apart.\",\"position\":3,\"name\":\"Using a\\u00a0small cookie scoop\\u00a0(about 1.5 tablespoon scoop) drop...\",\"url\":\"https:\\/\\/tidymom.net\\/lemon-cool-whip-cookies\\/#mv_create_171_3\"},{\"@type\":\"HowToStep\",\"text\":\"Bake for 10- 12 minutes or until slightly set and tops are cracked. Cool on pan for 5 minutes then transfer to wire racks to cool.\",\"position\":4,\"name\":\"Bake for 10- 12 minutes or until slightly...\",\"url\":\"https:\\/\\/tidymom.net\\/lemon-cool-whip-cookies\\/#mv_create_171_4\"}],\"video\":{\"@type\":\"VideoObject\",\"name\":\"LEMON BURST CAKE MIX COOKIES\",\"description\":\"Enjoy the burst of lemon flavor from these light, fluffy soft baked Lemon Whippersnaps.\",\"thumbnailUrl\":[\"https:\\/\\/mediavine-res.cloudinary.com\\/image\\/upload\\/s--y6s9ggrB--\\/ar_16:9,c_fill,f_auto,fl_lossy,q_auto\\/v1493320372\\/cwvfyu7zfnw55lr2kwfz.jpg\",\"https:\\/\\/mediavine-res.cloudinary.com\\/image\\/upload\\/s--zt58UOPN--\\/ar_4:3,c_fill,f_auto,fl_lossy,q_auto\\/v1493320372\\/cwvfyu7zfnw55lr2kwfz.jpg\",\"https:\\/\\/mediavine-res.cloudinary.com\\/image\\/upload\\/s--7yM5WFFv--\\/ar_1:1,c_fill,f_auto,fl_lossy,q_auto\\/v1493320372\\/cwvfyu7zfnw55lr2kwfz.jpg\"],\"contentUrl\":\"https:\\/\\/mediavine-res.cloudinary.com\\/video\\/upload\\/cojdmtaievbiozpe57ah.mp4\",\"duration\":\"PT55S\",\"uploadDate\":\"2022-08-31T21:46:05+00:00\"},\"keywords\":\" cake mix cookies, cool whip, home and recipe video, lemon\",\"nutrition\":{\"@type\":\"NutritionInformation\",\"calories\":\"63 calories\",\"carbohydrateContent\":\"14 grams carbohydrates\",\"cholesterolContent\":\"5 milligrams cholesterol\",\"fatContent\":\"1 grams fat\",\"fiberContent\":\"0 grams fiber\",\"proteinContent\":\"1 grams protein\",\"saturatedFatContent\":\"0 grams saturated fat\",\"servingSize\":\"1\",\"sodiumContent\":\"108 milligrams sodium\",\"sugarContent\":\"8 grams sugar\",\"transFatContent\":\"0 grams trans fat\",\"unsaturatedFatContent\":\"0 grams unsaturated fat\"},\"aggregateRating\":{\"@type\":\"AggregateRating\",\"ratingValue\":4.4,\"reviewCount\":\"96\"},\"url\":\"https:\\/\\/tidymom.net\\/lemon-cool-whip-cookies\\/\"}Lemon Cool Whip CookiesYield: 24-36 cookiesPrep Time: 15 minutes Cook Time: 12 minutes Total Time: 27 minutes PrintEnjoy the burst of lemon flavor from these light, fluffy soft baked Lemon Whippersnaps. 153554b96e
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